Draize Rabbit Eye Test Compatibility with Eye Irritation Thresholds in Humans: A Quantitative Structure-Activity Relationship Analysis

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Draize Eye Irritancy Test Rabbits

Household products, shampoos, pesticides, weed killers, even riot control gases, are just some of the products tested for irritancy in the eyes of conscious rabbits. The Draize test, introduced in 1944, is cruelly simple. The substance under test is sprayed or instilled into one eye of an albino rabbit. Generally no pain relief is given and the test often proceeds for 7 days during which the co...

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Colworth, United Kingdom as an in vitro alternative to the in vivo Draize rabbit eye test method for the assessment of eye irritation

The IRE test was developed by Burton and his colleagues at Unilever Research Laboratory, Colworth, United Kingdom as an in vitro alternative to the in vivo Draize rabbit eye test method for the assessment of eye irritation (Burton et al. 1981). In the IRE test method, liquid test substances are spread using a syringe and solids are pulverized and applied as a powder over the corneas of enucleat...

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The Rabbit Enucleated Eye Test

The use of isolated rabbit eyes for the assessment of eye irritation potential is a recognized alternative to the Draize eye irritation test, because it closely models the in vivo test system, but does not require the exposure to take place in the eyes of living animals. The procedure permits evaluation of undiluted test materials, i.e. as they could enter the in vivo eye test, and in this resp...

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Alternatives to In Vivo Draize Rabbit Eye and Skin Irritation Tests with a Focus on 3D Reconstructed Human Cornea-Like Epithelium and Epidermis Models

Human eyes and skin are frequently exposed to chemicals accidentally or on purpose due to their external location. Therefore, chemicals are required to undergo the evaluation of the ocular and dermal irritancy for their safe handling and use before release into the market. Draize rabbit eye and skin irritation test developed in 1944, has been a gold standard test which was enlisted as OECD TG 4...

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Surfactant-induced TRPV1 activity--a novel mechanism for eye irritation?

The pain receptor transient receptor potential vanilloid type 1 (TRPV1) has been reported as one of the key components in the pain pathway. Activation of the receptor causes a Ca2+ influx with secondary effects leading to neurogenic inflammation. Here we report specific activation of TRPV1 by detergent-containing hygiene products measured as intracellular Ca2+ influxes in stably TRPV1-expressin...

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ژورنال

عنوان ژورنال: Toxicological Sciences

سال: 2003

ISSN: 1096-0929

DOI: 10.1093/toxsci/kfg242